Courses
BTEC 2050 - Zebrafish Maintenance and Methodology
This course will give a broad introduction to zebrafish husbandry, maintenance, breeding, egg collection, and embryo microscopy. It will prepare students for advanced functional genomics coursework and research.
BTEC 3050 - Gene Knockout: RNA Interference & CRISPR
This course will introduce foundational techniques of gene knockout using the CRISPR/Cas9 System. Students will gain hands-on experience in a variety of techniques and carry out several experiments using CRISPR/Cas9. This class will prepare students for advanced functional genomics coursework and research.
BTEC 4050 - In Situ Hybridization
This course will introduce foundational techniques of in situ hybridization, and provide applied lab experiences in identifying abnormal phenotypes using in situ hybridization techniques. It will prepare students for advanced functional genomics coursework and research.
BTEC 4060 - Variant Validation in Zebrafish
This applied lab allows students to perform a functional genomics validation experiment in the zebrafish model. Students will be given a variant of unknown significance identified by hospital collaborators, and will carry out all steps required to validate the variant of unknown significance including: zebrafish mating and effective collection, microinjection, microscopic and in situ hybridization identification of phenotype, knockout rescue and variant validation.
